ab185033 goat polyclonal igg anti gfra2 r d systems  (R&D Systems)

Journal: NPJ Regenerative medicine

Article Title: Return of the Tbx5; lineage-tracing reveals ventricular cardiomyocyte-like precursors in the injured adult mammalian heart.

doi: 10.1038/s41536-023-00280-9

Figure Lengend Snippet: Fig. 1 Enrichment and optimization of in vitro mESC-derived CPC enrichment. A Representative microphotographs depicting stages of differentiation of murine ground state Tbx5Cre;R26ReYFP/eYFP mESC cultured under specified cardiomyocyte differentiation conditions, either as monolayers or as embryoid bodies. B Flow cytometric analysis using three surface markers Pdgfra, Kdr and Gfra2 on different days of cardiomyocyte differentiation indicates an CPC enrichment window between days 7 and 9. C The expression of Kdr is dynamic and defines two waves of CPC in the current differentiation protocol. N = 1–4. Error bars = SEM.

Article Snippet: Cells were labeled with the following antibodies: anti-human/mouse GFRA2 Polyclonal Goat IgG (R&D Systems, Cat no. AF429), rat monoclonal antiPDGFR alpha antibody conjugated with PE (Abcam, APA5, Cat no. ab93531), donkey polyclonal anti-goat IgG Alexa 405 conjugated with UV (Abcam, Cat no. ab175664), rat monoclonal IgG2b antimouse KDR-Alexa647 conjugated with APC (BioLegend, Cat no. 121910).

Techniques: In Vitro, Derivative Assay, Cell Culture, Expressing

Journal: NPJ Regenerative medicine

Article Title: Return of the Tbx5; lineage-tracing reveals ventricular cardiomyocyte-like precursors in the injured adult mammalian heart.

doi: 10.1038/s41536-023-00280-9

Figure Lengend Snippet: Fig. 5 CPC surface analysis of YFP+ cells. A Real-time PCR analysis of Yfp and Tbx5 transcripts in the ventricles of the adult heart in different time-points. B Flow cytometry acquisition of YFP+ cells from different time-points following cardiac injury. N = 3–6 hearts. C, D Representative two- and three-dimensional graphs from flow cytometric analysis of Pdgfra+Kdrlow/+Gfra2+ adult, postnatal days 6 and 9 heart and their co- expression with YFP (Tbx5-tracing), Sca-1 and c-Kit. N = 9. Key-CP; cardiomyocyte precursor, CM; cardiomyocyte, EC; endothelial cell. E YFP+ cells were collected 7 days after chemical injury and cultured in CM differentiating conditions for 5 and 8 days. Only YFP+ from the injured heart were able to differentiate into CM-like cells. Measurement of YFP+/cTnT+ mononucleated and binucleated cells 5 and 8 days in vitro culture. N = 5–6 independent cultures. Mann–Whitney test, p = 0.0519 (ns = not statistically significant) for D5 binucleated/ mononucleated ratio.

Article Snippet: Cells were labeled with the following antibodies: anti-human/mouse GFRA2 Polyclonal Goat IgG (R&D Systems, Cat no. AF429), rat monoclonal antiPDGFR alpha antibody conjugated with PE (Abcam, APA5, Cat no. ab93531), donkey polyclonal anti-goat IgG Alexa 405 conjugated with UV (Abcam, Cat no. ab175664), rat monoclonal IgG2b antimouse KDR-Alexa647 conjugated with APC (BioLegend, Cat no. 121910).

Techniques: Real-time Polymerase Chain Reaction, Flow Cytometry, Expressing, Cell Culture, In Vitro, MANN-WHITNEY

Journal: NPJ Regenerative medicine

Article Title: Return of the Tbx5; lineage-tracing reveals ventricular cardiomyocyte-like precursors in the injured adult mammalian heart.

doi: 10.1038/s41536-023-00280-9

Figure Lengend Snippet: Fig. 6 The transcriptome of YFP+ cell sub-clusters resembles that of CM precursors. A t-SNE dimensionality analysis and data store tree of EdgeR p < 0.05 after Benjamimi and Hochberg correction (8749 DEGs) of YFP+ (green) and Pdgfra+ (blue) interstitial adult heart cells. YFP+ cells could be divided into at least three sub-clusters. Single cells examined were obtained from 3 adult injured D7 hearts. B Heatmaps depicting expression of Tbx5, Kdr, Gfra2 and Pdgfra and cardiac fibroblast DEGs in YFP+ (green) and uninjured Pdgfra+ (purple) adult heart cells. C t-SNE dimensionality, and volcano plot of DEGs between the two major YFP+ sub-clusters (1091 DEGs). D Heatmap clustering analysis of CM-relevant genes within the DEG list that showed prominent expression differences between the two YFP+ sub-clusters.

Article Snippet: Cells were labeled with the following antibodies: anti-human/mouse GFRA2 Polyclonal Goat IgG (R&D Systems, Cat no. AF429), rat monoclonal antiPDGFR alpha antibody conjugated with PE (Abcam, APA5, Cat no. ab93531), donkey polyclonal anti-goat IgG Alexa 405 conjugated with UV (Abcam, Cat no. ab175664), rat monoclonal IgG2b antimouse KDR-Alexa647 conjugated with APC (BioLegend, Cat no. 121910).

Techniques: Expressing